GLI1

GLI family zinc finger 1
PDB rendering based on 2gli.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2GLI, 4BLB, 4KMD
Identifiers
Symbols	GLI1 ; GLI
External IDs	OMIM: 165220 MGI: 95727 HomoloGene: 3859 ChEMBL: 5461 GeneCards: GLI1 Gene
Gene ontology Molecular function • DNA binding • chromatin binding • RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity • protein binding • microtubule binding • transcription regulatory region DNA binding • metal ion binding Cellular component • nucleus • nucleoplasm • cytoplasm • cytosol • intracellular membrane-bounded organelle • primary cilium • ciliary tip • ciliary base Biological process • osteoblast differentiation • transcription from RNA polymerase II promoter • smoothened signaling pathway • spermatogenesis • ventral midline development • positive regulation of cell proliferation • regulation of smoothened signaling pathway • epidermal cell differentiation • dorsal/ventral pattern formation • proximal/distal pattern formation • cerebellar cortex morphogenesis • smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation • pituitary gland development • lung development • regulation of osteoblast differentiation • positive regulation of DNA replication • positive regulation of smoothened signaling pathway • positive regulation of transcription, DNA-templated • positive regulation of transcription from RNA polymerase II promoter • digestive tract morphogenesis • notochord regression • negative regulation of canonical Wnt signaling pathway Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	2735	14632
Ensembl	ENSG00000111087	ENSMUSG00000025407
UniProt	P08151	P47806
RefSeq (mRNA)	NM_001160045	NM_010296
RefSeq (protein)	NP_001153517	NP_034426
Location (UCSC)	Chr 12: 57.46 – 57.47 Mb	Chr 10: 127.33 – 127.34 Mb
PubMed search	[1]	[2]

Gli1 is a protein originally isolated in human glioblastoma.

Overview

The Gli proteins are the effectors of Hedgehog (Hh) signaling and have been shown to be involved in cell fate determination, proliferation and patterning in many cell types and most organs during embryo development. In the developing spinal cord the target genes of Gli proteins, that are themselves transcription factors, are arranged into a complex gene regulatory network that translates the extracellular concentration gradient of Sonic hedgehog into different cell fates along the dorsoventral axis.

The Gli transcription factors activate/inhibit transcription by binding to Gli responsive genes and by interacting with the transcription complex. The Gli transcription factors have DNA binding zinc finger domains which bind to consensus sequences on their target genes to initiate or suppress transcription. Yoon showed that mutating the Gli zinc finger domain inhibited the proteins effect proving its role as a transcription factor. Gli proteins have an 18-amino acid region highly similar to the α-helical herpes simplex viral protein 16 activation domain. This domain contains a consensus recognition element for the human TFIID TATA box-binding protein associated factor TAFII31. Other proteins such as Missing in Metastasis (MIM/BEG4) have been shown to potentiate the effects of the Gli transcription factors on target gene transcription. Gli and MIM have been shown to act synergistically to induce epidermal growth and MIM + Gli1 overexpressing grafts show similar growth patterns to Shh grafts.

Gli Family

There are three members of the family; Gli1, Gli2 and Gli3 which are all transcription factors mediating the Hh pathway. The GLI1, GLI2, and GLI3 genes encode transcription factors which all contain conserved tandem C2-H2 zinc finger domains and a consensus histidine/cysteine linker sequence between zinc fingers. This Gli motif is related to those of Kruppel which is a Drosophila segmentation gene of the gap class. In transgenic mice, mutant Gli1 lacking the zinc fingers does not induce Sonic Hedgehog (Shh) targets. The conserved stretch of 9 amino acids connects the C-terminal histidine of one finger to the N-terminal cysteine of the next. The GLI consensus finger amino acid sequence is [Y/F]JXCX3GCX3[F/Y]X5LX2HX4H[T/S]GEKP. The Gli1 and Gli2 protein zinc finger DNA binding domain have been shown to bind to the DNA consensus GLI binding site GACCACCCA.

Gli Proteins transcriptional regulation is tissue specific for many targets. For example, Gli1 in primary keratinocytes upregulates FOXM1 whereas in mesenchymal C3H10T1/2 cells it has been shown to upregulate platelet-derived growth factor receptor PDGFRa.

Human Gli1 encodes a transcription activator involved in development that is a known oncogene. It has been found that N-terminal regions of Gli1 recruit histone deacetylase complexes via SuFu, which are involved in DNA folding in chromosomes. This may negatively regulate transcription indicating Gli1 could act as transcriptional inhibitor as well as an activator. The human GLI1 promoter region is regulated by a 1.4 kb 5’ region including a 5’ flanking sequence, an untranslated exon and 425bp of the first intron. Numerous proteins such as Sp1, USF1, USF2, and Twist are also involved in Gli1 promoter regulation. During mouse embryo development Gli1 expression can be detected in the gut mesoderm, ventral neural tube, ependymal layer of the spinal cord, forebrain, midbrain, cerebellum, and in sites of endochondral bone formation. Some of the downstream gene targets of human Gli1 include regulators of the cell cycle and apoptosis such as cyclin D2 and plakoglobin respectively. Gli1 also upregulates FoxM1 in BCC. Gli1 expression can also mimic Shh expression in certain cell types.

Isolation

GLI1 was originally isolated from a glioma tumour and has been found to be up regulated in many tumors including muscle, brain and skin tumors such as Basal cell carcinoma (BCC). Shh and the Gli genes are normally expressed in hair follicles, and skin tumours expressing Gli1 may arise from hair follicles. The level of Gli1 expression correlates with the tumor grade in bone and soft tissue sarcomas. Transgenic mice and frogs overexpressing Gli1 develop BCC like tumours as well as other hair follicle-derived neoplasias, such as trichoepitheliomas, cylindromas, and trichoblastomas. Expression of Gli1 in the embryonic frog epidermis results in the development of tumours that express endogenous Gli1. This suggests that overexpressed Gli1 alone is probably sufficient for tumour development Mutations leading to the expression of Gli1 in basal cells are thus predicted to induce BCC formation.

Interactions

GLI1 has been shown to interact with:

• SAP18,
• STK36,
• SUFU, and
• ZIC1.

External links

• Gli1 protein at the US National Library of Medicine Medical Subject Headings (MeSH)