TWF1

Twinfilin actin binding protein 1
250px PDB rendering based on 1m4j.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1m4j, 2d8b, 2hd7
Identifiers
Symbols	TWF1 ; A6; PTK9
External IDs	OMIM: 610932 MGI: 1100520 HomoloGene: 48140 GeneCards: TWF1 Gene
Gene ontology Molecular function • actin monomer binding • protein tyrosine kinase activity • ATP binding • phosphatidylinositol-4,5-bisphosphate binding Cellular component • cytoplasm • cell-cell junction • focal adhesion • actin cytoskeleton • myofibril • filopodium • ruffle membrane • perinuclear region of cytoplasm Biological process • negative regulation of actin filament polymerization • sequestering of actin monomers • regulation of actin phosphorylation Sources: Amigo / QuickGO
RNA expression pattern
File:PBB GE TWF1 201745 at tn.png
File:PBB GE TWF1 214007 s at tn.png
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	5756	19230
Ensembl	ENSG00000151239	ENSMUSG00000022451
UniProt	Q12792	Q91YR1
RefSeq (mRNA)	NM_001242397	NM_008971
RefSeq (protein)	NP_001229326	NP_032997
Location (UCSC)	Chr 12: 43.79 – 43.81 Mb	Chr 15: 94.58 – 94.59 Mb
PubMed search	[1]	[2]

Twinfilin-1 is a protein that in humans is encoded by the TWF1 gene. This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.

Model organisms

Twf1 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Normal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Plasma immunoglobulins	Normal
Haematology	Normal
Micronucleus test	Normal
Heart weight	Normal
Eye Histopathology	Normal
Salmonella infection	Normal
Citrobacter infection	Normal
All tests and analysis from

Model organisms have been used in the study of TWF1 function. A conditional knockout mouse line, called Twf1^{tm1a(EUCOMM)Wtsi} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. Twenty three tests were carried out on mutant mice, but no significant abnormalities were observed.