Glycoside hydrolase family 5
| Cellulase (glycosyl hydrolase family 5) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | Cellulase | ||||||||
| Pfam | PF00150 | ||||||||
| Pfam clan | CL0058 | ||||||||
| InterPro | IPR001547 | ||||||||
| PROSITE | PDOC00565 | ||||||||
| SCOP | 2exo | ||||||||
| SUPERFAMILY | 2exo | ||||||||
| OPM superfamily | 125 | ||||||||
| OPM protein | 2osx | ||||||||
| CAZy | GH5 | ||||||||
|
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In molecular biology, glycoside hydrolase family 5 is a family of glycoside hydrolases.
Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy(http://www.cazy.org/Glycoside-Hydrolases.html) web site,[4] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[5]
Glycoside hydrolase family 5 CAZY GH_5 comprises enzymes with several known activities including endoglucanase (EC 3.2.1.4); beta-mannanase (EC 3.2.1.78); exo-1,3-glucanase (EC 3.2.1.58); endo-1,6-glucanase (EC 3.2.1.75); xylanase (EC 3.2.1.8); endoglycoceramidase (EC 3.2.1.123).
The microbial degradation of cellulose and xylans requires several types of enzymes. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family A[6] or as the glycosyl hydrolases family 5.[7] One of the conserved regions in this family contains a conserved glutamic acid residue which is potentially involved[8] in the catalytic mechanism.
In a recent study using Molecular Dynamics simulations, a considerable correlation between thermal stability and structural rigidity of members of family 5 with solved structures has been proved.[9]
References
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- ↑ Lua error in package.lua at line 80: module 'strict' not found.
- ↑ Bairoch, A. "Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT". 1999.
- ↑ Henrissat, B. and Coutinho P.M. "Carbohydrate-Active Enzymes server". 1999.
- ↑ CAZypedia, an online encyclopedia of carbohydrate-active enzymes.
- ↑ Lua error in package.lua at line 80: module 'strict' not found.
- ↑ Lua error in package.lua at line 80: module 'strict' not found.
- ↑ Lua error in package.lua at line 80: module 'strict' not found.
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